At the Florida headquarters near Tampa, we maintain a complete nematology facility including a dedicated laboratory, greenhouses, field micro-plots, advanced microscopy, and pure cultures of important pest nematode species. Also, several of our California and Florida farms have in-ground micro-plots that isolate nematode populations for close monitoring of responses to test substances. In these vessels, different soil types, selected nematode species, and individual crop plants are studied with minimal variability and hence experimental repeatability. These methods also minimize experimental cost and can answer very specific questions reguarding efficacy, longevity, potential for phytotoxicity, nematode life stage mortality, and specific plant responses such as plant growth, tolerance to foliar pests, and crop yield.
Pacific Ag Research fumigation equipment being used to remove soil-borne pests such as nematodes
The nematology lab at Florida Ag Research
Some of our nematology research capabilities include:
- Extraction Methodologies: Berhmen funnel technique, and several variations of Sucrose Density Extraction methods. State-of-the-art Nematology Research laboratory with centrifuges, dissecting, compound and inverted microscopes, sampling equipment and soil filtration systems.
- Pure cultures:Pure cultures of important plant parasitic nematodes are maintained on specific hosts targeted for experimentation. Pure cultures are maintained for Sting nematodes ( Belonolaimus longicaudatus), Root Knot nematode ( Meloidogyne spp i.e. M. arenaria M. incognita, M. javanica, M. graminis , M. hapla), Lesion nematodes (Pratylenchus spp.), Lance Nematodes (Hoplolaimus spp), Spiral nematode (Helicotylenchus spp.), citrus nematode (Tylenchulus semipenetrans).
- Bio assays include both in vitro and in vivo: methods for determining individual species sensitivities to specific test substances.
- Greenhouse research capabilities: include over 1.5 acre of covered glasshouse, 2 acre of shade houses managed by technicians trained in nematode culture.
- Open field micro plots: Pacific Ag Group has microplots in both eastern and western research facilities to support efficacy testing for nematodes of economic importance. Open field planting dedicated to nematode biology and control total over 100 acres split between Eastern and Western stations. These fields are used for product demonstrations and replicated small research plots where nematode species are uniformly distributed and complete historical records are maintained.
- Application equipment:Broadcast and raised bed shanked injection, drip chemigation, preplant granular and liquid drench treatments, and post-planting in-season drenches and other techniques required by sponsors and mastered by Pacific Ag technicians. Evaluations include several growth indices including analysis with digitized leaf area, visual ratings conducted in-season in open field plots, plant color determination, and numerous harvest parameters that can demonstrate even subtitle effects of nematode feeding. Research reports include photographic documentation of species, morphological changes in treated specimens, as well as a complete work up of experimental results in both text and graphical presentations.